Osteoclast-derived exosomal miR-5134-5p interferes with alveolar bone homeostasis by targeting the JAK2/STAT3 axis
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https://www.ncbi.nlm.nih.gov/sra/SRP415609
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The specific mirnas in the exosomes of mature osteoclasts were found by exosome sequencing, and their functions were identified by acting on osteoblasts to observe the differentiation and osteogenesis of osteoblasts Overall design: Source: RAW264.7. In the T75 culture bottle, 100W cells were cultured in Gibco DMEM+10%FBS for 24h, then the supernatant was abandoned. After washing with PBS, the supernatant was changed to a-MEM+10%FBS (control group), a-MEM+10%FBS+50ng/mlRANKL (induction group), respectively. Cells in the supernatant were removed by centrifugation at 300g for 9min at 4 ° C and 2000g for 20min, and the precipitate was discarded
创建时间：
2023-07-18
