Small RNA-seq analysis shows reduced tRNA levels in cdk1-as1 mutants

Purpose: Determine whether Cdk1 only regulates a specific subset of tDNAs, or whether Cdk1 has a more global impact on tDNA expression; Method: We study the expression of tRNA genes in WT cells and cdk1-as1 mutants during S phase by small-RNAseq; Result: We found that expression of at least 77% of all tDNAs is significantly downregulated in S-phase in the cdk1-as1 mutant compared with the WT strain; Conclussions: Cdk1 promotes transcription of the majority of tRNA genes during the cell cycle.研究目的：明确周期蛋白依赖性激酶1（Cdk1）是否仅调控特定子集的转运RNA基因（tDNA），抑或其对tDNA的表达具有更广泛的全局影响；研究方法：通过小RNA测序（small-RNAseq）分析S期野生型（Wild Type，WT）细胞与cdk1-as1突变体中的tRNA基因表达水平；研究结果：相较于野生型菌株，cdk1-as1突变体在S期内至少77%的tDNA表达量显著下调；研究结论：Cdk1在细胞周期进程中促进大多数tRNA基因的转录