Microcurrent-Mediated Modulation of Myofibroblasts for Cardiac Repair and Regeneration

Myofibroblasts are one of the key cell types in cardiac injuries, leading to scar tissue formation and reduced heart function. To investigate the impact of microcurrent treatment on these cells, primary rat cardiac fibroblasts were initially differentiated into myofibroblasts. Subsequently, the differentiated myofibroblasts were subjected to microcurrent treatment at 1 and 2 µA/cm2 direct current (DC) with and without polarity reversal. Microcurrent treatments resulted in distinct transcriptional signatures and improved cell behavior. The observations show signs of microcurrent-mediated reversal of myofibroblast phenotype, possibly reducing cardiac fibrosis, and providing insights for cardiac tissue repair.肌成纤维细胞（Myofibroblasts）是心脏损伤过程中的关键细胞类型之一，可介导瘢痕组织形成并导致心脏功能减退。为探究微电流治疗对该类细胞的影响，研究人员首先将原代大鼠心脏成纤维细胞诱导分化为肌成纤维细胞。随后，对已分化的肌成纤维细胞施加强度为1和2 µA/cm²的直流（DC）微电流处理，并设置有无极性反转的实验组。实验结果显示，微电流处理可诱导产生独特的转录特征，并改善细胞行为。观测数据表明，微电流可介导肌成纤维细胞表型逆转，或可减轻心脏纤维化，为心脏组织修复相关研究提供了新的思路与理论支撑。