Single cell RNA-sequencing analysis of astrocyte-to-neuron conversion in 5xFAD mouse hippocampus

Collectively, our findings demonstrate that NeuroD1 significantly alters the transcriptomic landscape of astrocytes, suggesting a role for NeuroD1 in neuronal fate reprogramming. 5xFAD mouse Hippocampi were injected with AAV9 and extracted from control and NeuroD1 treated mice at 3 weeks post injection (n = 3 mice per group). Hippocampi were dissociated, and single-cell suspensions were prepared and isolated using a microfluidic chip, followed by scRNA-seq processing.