Combination assays results and amino acid-limited conditions (Fig 5, 6E–L and S9 Fig).

(A) Combination assays of parasites in the presence of increasing concentrations of PPQ and a constant concentration of E64, pepA, ConA, or CCCP measured as AUC. (B) Parasites were cultured either in regular or amino acid-limited media and exposed to increasing concentrations of PPQ and the AUC was measured. (C) EC50 for ConA and CCCP. (D) Combination assays in the presence of increasing concentrations of CQ and a constant concentration of CCCP measured as EC50. (XLSX)