Effect of CSPG stimulation on Fibcd1 WT and KO primary hippocampal neurons

To investigate if Fibcd1 mediates CSPG signalling to neurons, we established a primary mouse hippocampal neuron culture plated +/- a coating of CSPGs. After determining that Fibcd1 KO neurons are partially resistant to CSPG signals, we sought to identify the transcriptional finger print of CSPG-Fibcd1 signalling by nulk RNA-seq. Overall design: Primary hippocampal neurons were isolated from Fibcd1 WT and KO mice and plated on coverslips +/- a coating of CSPGs