Transcriptome of ASFV BA71V during Early and Late Infection of Vero cells

African Swine Fever Virus causes haemorrhagic fever in domestic pigs and presents the biggest global threat to animal farming in recorded history. Despite its importance, very little is known the mechanisms and temporal regulation of transcription in ASFV. Here we report the first detailed viral transcriptome analysis of ASFV during early and late infection of Vero cells. In addition to total RNA sequencing, we have characterised the transcription start sites and transcription termination using CAGE-seq and 3' RNA-seq, respectively, revealing the distinct DNA consensus motifs of early and late promoters, as well as the sequence determinants for transcription termination.