Comparative expression data between intestinal epithelium of FSPCre-PPARB -/- and PPARB flox/flox. Mus musculus

Stroma secretes a multitude of regulatory signals that are involved in mediating intestinal development. Ablation of PPARB expression in fibroblastic cells results in modulation of oxidative stress signals that lead to altered intestinal morphology and gene expression profiles Through gene chip expression profiling, we seek to identify key changes in intestinal mucosal gene expression profiling between FSPCre-PPARB flox/flox and PPARB flox/flox mice. Overall design: Expression profiling of FSPCre-PPARB KO colon mucosa, FSPCre-PPARB KO ileum mucosa, PPARB floxed colon mucosa, PPARB floxed ileum mucosa. Intestines were harvested from 4 to 6 week old C57BL6/J mice and prepared for histological staining. Intestinal mucosal tissue were microdissected from stained histological slides using Zeiss PALM III laser disection microscope.