H5N8 Influenza A Virus Genomes: Codon Usage Bias and Spatiotemporal Distribution n = 5219

This dataset features 5,219 complete H5N8 influenza A virus sequences from NCBI, processed through local bioinformatics workflows.
Sequences were filtered for completeness, aligned with MAFFT (--parttree --retree 1), and trimmed.
Codon-level metrics including ENc, CAI (human and avian), GC3, RSCU, CPB, and PR2 were computed per sequence.
Collection year and country were parsed from headers. Includes aligned FASTA, codon metrics CSV, QC summaries, and publication-ready visualizations.
CODON USAGE SUMMARY: H5N8
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Total sequences: 5,219
Valid CDS: 1,703 (32.6%)
Valid CAI: 1,703 (32.6%)

METRIC STATISTICS (Mean ± SD):
ENc (Effective Number of Codons): 35.697 ± 1.497
CPB (Codon Pair Bias): 5.186 ± 1.103
CAI_human: 3.416 ± 0.137
CAI_avian: 3.436 ± 0.141
GC3: 0.449 ± 0.031
PR2_A (A3/(A3+T3)): 0.564 ± 0.043
PR2_G (G3/(G3+C3)): 0.531 ± 0.032

INTERPRETATION:
→ Strong codon usage bias (ENc < 40)
→ Similar adaptation to human/avian hosts
Data processed by: [e-mail] for upcoming journal Article.