Dataset on Cytosolic Hyper-replication of Salmonella in Cell Models Representative of Salmonellosis, Assessed by Imaging, Flow Cytometry, and Bacteriology

The data presented here are the raw data used to construct the figure for a draft submitted for publication titled : Cytosolic hyper-replication of Salmonella occurs in all physiologically relevant host cell types, with variable T3SS-1 involvement. Figure 1 : Raw Images (TIFF imageJ) used to illustrate that Hyper-replicating Salmonella occur in diverse endothelial, fibroblast and immune cell lines, in addition to epithelial cells. Figure 2 : RAW data obtained by quantification of area of cell exposed to Salmonella hyper replication allow to conclude that Hyper-replicating Salmonella are detected as early as 6 hours post-infection. Figure 3 : RAW Images and RAW data used to done Quantification and visualization of late-stage cytosolic Salmonella replication in AML12 cells Figure 4 : RAW Images and RAW data used to support our hypothesis that The hyper-replication phenotype does not occur in all epithelial, endothelial, and immune cell types. Figure 5 : RAW Images and RAW data used to support our hypothesis that sfGFP-positive Salmonella remains associated with LAMP-1 in AML12, HBMEC and stimulated THP-1 cells at 8h p.i. Figure 6 : RAW data used to support that Bacterial load has no impact on the intracellular fate of Salmonella. Figure 7 : RAW data used to support that The intracellular behavior remains unchanged regardless of the Salmonella serotype or strain used. Figure 8 : RAW data used to support that The ability to hyper-replicate in the cytosol does not depend on Salmonella entry mechanism used Figure 9 : RAW Images and RAW data used to support that Cytosolic hyper-replication can be dependent or independent of the T3SS-1 Figure S1: RAW data of flow cytometry used to support Flow cytometry analyses of HeLa cells infection by Salmonella Typhiumrium 14028. Figure S2: RAW data obtained by quantification of area of cell exposed to Salmonella and used to support that Salmonella proliferates over time during infection. Figure S3 : RAW data obtained by quantification of area of cell exposed to Salmonella and used to support that There is no defect in bacterial replication over time within the AML12 hepatocyte cell line Figure S4 : RAW data obtained by quantification of area of cell exposed to Salmonella and used to support that There is no defect in bacterial replication over time within cellular models exhibiting vacuolar destabilization Figure S5: RAW data obtained by quantification of area of cell exposed to Salmonella and used to support thatSalmonella SL1344 and LA5 strains show higher infection rates compared to the Salmonella 14028 strain accross the various cell type tested