Supplementary Material for: Title: Hans-based cell of origin is not of prognostic significance in DLBCL patients; Retrospective single center study conducted in 326 RCHOP treated patients

Background: Diffuse large B-cell lymphoma (DLBCL) is a highly aggressive lymphoma with dismal outcome after disease progression. Individual risk assessment can stratify patients into different treatment strategies. Methods: We retrospectively collected data from 326 DLBCL patients treated in a single center with RCHOP from 01/2000 to 06/2023. Immunohistochemistry by Hans algorithm was used to classify patients according to cell of origin (COO). The Kaplan-Meier estimator of survival and Cox regression analysis were used. Results: Time to progression (TTP) and overall survival (OS) were not different according to COO. Univariate analysis reveals international prognostic index (IPI), b2-microgloboulin ≥ 3,5 mg/L, bulky disease and abnormal LDH, but not Han’s defined COO, as the strongest predictors for progression. IPI score in multivariate analysis was the only prognostic factor for OS and together with high b2-microglobulin levels were independently prognostic factors for TTP. Accordingly, b2-microglobulin ≥ 3,5 mg/L was an independent prognostic factor for progression both in GC (HR 0,249 [(95% CI: 0,087-0,649), p=0,004] and non-GC DLBCL patients [HR 0,380 (95% CI: 0,177-0,813), p=0,011]. Conclusions: Cell of origin according to Hans Index is not a significant prognostic factor for DLBCL patients but b2 microglobulin ≥ 3,5 mg/L and IPI 2-5 in both GC and non-GC patients can predict individually a higher risk for progression.