Gene expression profiling of RasV12-induced brain tumors and tumor-associated macrophages

The goal of this study is to profile gene expression patterns of RasV12-induced brain tumors and tumor-associated macrophages (TAM) in the cerebellum. Ras activation in Msi1+ cells induced tumor formation in the cerebellum of Msi1-CreER;RasV12 mice (Ras). GFP+ tumor cells and CD11b+ phagocytes were isolated from the cerebellum of Ras mice at 3 weeks and used for gene expression analysis. GFP+ cells and CD11b+ phagocytes isolated from the cerebellum of Msi1-CreER;GFP mice (Cont) were used as control. mRNA profiles of GFP+ cells and CD11b+ cells in cont and Ras mice were generated by deep sequencing, in triplicate, using Illumina HiSeq3000.