Preparation and application of peptide molecular imprinted material based on mesoporous metal-organic framework

Fig.2 XRD patterns of (a) simulated UiO-66-NH2, (b)microporous UiO-66-NH2, (c)mesoporous UiO-66-NH2 and (d) mesoporous MIP@UiO-66-NH2.
Fig.3 SEM images of (a)microporous UiO-66-NH2, (b) mesoporous UiO-66-NH2 and (c) mesoporous MIP@UiO-66-NH2; TEM images of (d) microporous UiO-66-NH2 (e) mesoporous UiO-66-NH2 and (f) mesoporous MIP@UiO-66-NH2
Fig.4 N2 sorption isotherms of (a) UiO-66-NH2 (b) UiO-66-NH2 after decarboxylation (c) mesoporous MIP@UiO-66-NH2 and pore sizes distribution of (d) UiO-66-NH2 (e) UiO-66-NH2 after decarboxylation (f) mesoporous MIP@UiO-66-NH2
Fig.5 The effects of pore size and dosage of MOFs on the adsorption capacity of [e-mail] conditions: mGSH=100 mg, mAM=0.72 g, mNVP=0.56 g, mNMBA=10 mg, and mDMDAAC=0.14 g; adsorption conditions:cGSH,0=0.1 g·L-1, V=1 mL, mMIP=1 mg, t=4 h, pH=7.00, T=4 ℃; eluent : CH3OH/HAc (9/1, v/v)
Fig.6 The effect of the ratio of cross-linking agents and functional monomers on the adsorption capacity of [e-mail] conditions: mGSH=100 mg, mmesoporous UiO-66-NH2=100 mg, other conditions see Fig.5
Fig.7 The effect of eluent on the adsorption capacity of MIP@UiO-66-NH2.
Polymerization conditions: mGSH=100 mg, mmesoporous UiO-66-NH2=100 mg, mAM=0.72 g, mNVP=0.56 g, mNMBA=2 mg, mDMDAAC=0.028 g; other conditions see Fig.5
Fig.8 (a)Adsorption isotherm, (b) the Langmuir model, (c) the Freundlich model of [e-mail] conditions see Fig.5; polymerization conditions see fig.7, eluent: 1% SDS/10% HAc (w/v)
Fig.9 Kinetic adsorption curves and kinetic model fitting curves of (a) Pseudo-first order model and (b) Pseudo-second order model of MIP@UiO-66-NH2 at different temperatures. Adsorption conditions: t=1, 2, 5, 10, 20, 30, 40, 50, 60, 120 min, T=4℃, 25℃, 37℃, other conditions see Fig.5; polymerization conditions see Fig.7; eluent:1% SDS/10% HAc (w/v)
Fig.10 The MALDI-TOF mass spectra:(a) the remaining solution after adsorption (the loading condition: 0.10 g·L-1of GSH and 0.10 g·L-1 BSA tryptic digest) (b) the obtained eluent, (c) the remaining solution after adsorption (the loading condition:0.10 g·L-1 of GSH and 1.0 g·L-1 BSA tryptic digest),(d) the obtained eluent (★indicates GSH)
Fig.11 The MALDI-TOF mass spectra:(a) the remaining solution after adsorption (the loading condition: 500 μL 0.10 g·L-1of GSH and 500 μL skim milk tryptic digest) (b) the obtained eluent, (c) the remaining solution after adsorption (the loading condition: 500 μL 0.10 g·L-1of GSH and 500 μL egg white tryptic digest),(d) the obtained eluent (★indicates GSH)