m6A epitranscriptome regulates axonogenesis and reprogramming of retinal ganglion cells [RNA-seq]

induced retinal ganglion cells (iRGCs) is an excellent system to generate RGC-like neurons for translational and theoretical studies. N6-methyladenosine (m6A) is the most prevalent internal modification in mRNAs. Using this iRGC system, we demonstrated that Mettl3, the core component of the m6A-methyltransferase complex, promotes iRGC axon development and the final iRGC reprogramming outcome. Overall design: mouse embryonic fibroblasts (MEFs) infected with Ascl1/Brn3b/Islet1 overexpressing lentiviruses of different treatment groups were collected at day 5 after induction, and processed for RNA-seq.