RiboTag and bulk RNA sequencing using AAV-Control and AAV-shCsde1 infected sensory neurons.

Peripheral neurons can regenerate after nerve injury. To investigate the role of the RNA-binding protein CSDE1 and its interaction with ribosomal protein mRNAs in regenerating neurons, we performed total RNA and RiboTag sequencing analysis using AAV-Control or AAV-shCsde1 infected sensory neurons using RiboTag mice, RPL22HA+/+;Advillin-Cre+. Overall design: RiboTag sequencing data from cultured sensory neurons infected with AAV-Control and AAV-shCsde1. Preconditioning through sciatic nerve injury was conducted on mice before the neuron culture to activate their regenerative state.