Expression data from NCI-H1299 and NCI-H1299/CDDP cells

Non-small cell lung cancer (NSCLC) patients are prone to drug resistance during chemotherapy. Therefore, in order to compare the changes in the gene expression profiles of NSCLC cells before and after drug resistance, we constructed cisplatin-resistant cells (NCI-H1299/CDDP), and compared the gene expression profiles with those of the parental NCI-H1299 cells. We used microarrays to study in detail the global gene expression changes before and after drug resistance in NSCLC cells and identified genes that were up- or down-regulated during this process. NCI-H1299 and NCI-H1299 cells were cultured with RPMI 1640 medium containing 10% fetal bovine serum and 1% penicillin-streptomycin , then were incubated in 37 °C cell incubator containing 5% CO2.When the cell density reached 90-100%, the cells were collected and total RNA was extracted