PIMT/NcoA6IP deletion in the mouse heart causes delayed cardiomyopathy attributable to perturbation in energy metabolism

Global gene expression analysis reveals that PIMT regulates many genes involved in energy metabolism, calcium signaling, and oxidative phosphorylation in myocardium. RNA samples were prepared from hearts of 5 control PIMT floxed mice (PIMTfl/fl) and 5 Cre-loxP-mediated cardiomyocyte-specific deletion of PIMT (csPIMT-/-) mice. RNA were pooled and subjected to RNA-seq analysis.全基因表达分析结果表明，PIMT可调控心肌组织中参与能量代谢、钙信号转导及氧化磷酸化过程的众多基因。本实验从5只对照PIMT floxed小鼠（PIMTfl/fl）以及5只经Cre-loxP介导的心肌细胞特异性PIMT敲除（csPIMT-/-）小鼠的心脏中制备RNA样品，将RNA样品混合后开展RNA测序（RNA-seq）分析。