Single-Cell RNAseq Analysis of Murine Fate-Mapped Tsc1+/+ and Tsc1-/- Aortic Smooth Muscle Cells

We characterized the transcriptomic effects at the single cell level of chronic mTOR activation in aortic SMCs using a conditional genetic model to delete Tsc1 under control of a smooth muscle-specific Myh11 promoter Myh11-CreERT2.mT/mG (Tsc1+/+) and Tsc1fl/fl.Myh11-CreERT2.mT/mG (Tsc1−/−) male mice were treated with tamoxifen at 1.5 wk, the thoracic aortas were procured and enzyme digested at 24 wk, single, viable, GFP+ SMCs were isolated by FACS, and analyzed by single-cell RNAseq using the 10x system.