Single-nucleus profiling highlights the all-brain echinoderm nervous system

Metazoans comprise diverse tissues and cell types, each essential for the survival of the organism. Most of these types are established early in embryogenesis and persist into adulthood. In indirectly developing sea urchins, however, the continuity between embryonic and adult stages is interrupted by a planktonic larval stage that undergoes complete metamorphosis. While gene regulatory networks controlling embryonic and larval lineages are well studied, the molecular and morphological identities of post-metamorphic cell types remain poorly understood. Here, we reconstructed the cell type atlas of post-metamorphic Paracentrotus lividus juveniles using single-nucleus transcriptomics, revealing conservation of regulatory mechanisms. We identified signatures of eight distinct cell type groups and analyzed 29 neuronal families, including 15 unique photoreceptor types. By combining transcriptomics, spatial analysis, and ultrastructure, we identified vertebrate neuronal and opsin homologues ex..., , , # Single-nucleus profiling highlights the all-brain echinoderm nervous system

Access this dataset on Dryad: [https://doi.org/10.5061/dryad.0k6djhbdf](https://doi.org/10.5061/dryad.0k6djhbdf)

This repository contains scripts and Supplementary Materials related to the analyses

## Description of the data and file structure

This repository contains scripts used for the manuscript \"Single Nucleus Profiling Highlights the All-Brain Echinoderm Nervous System\" by Periklis Paganos, Jack Ullrich-Lüter, Alba Almazán, Danila Voronov, Jil Carl, Anne-C. Zakrzewski, Berit Zemann, Maria Lorenza Rusciano, Tiphaine Sancerni, Maria Schauer, Oğuz Akar, Filomena Caccavale, Maria Cocurullo, Giovanna Benvenuto, Jenifer Carol Croce, Carsten Lüter, Maria Ina Arnone. It also contains files associated with the mapping of snRNA-seq data (pl_cellranger_forced.sh), the analyses reported in the manuscript (Clustering_analysis_2wpm_Plividus_juvenile.R), and the larva versus juvenile cross-species comparison (Pl_cr...