Single-cell RNA sequencing of developing mouse small intestine

The mammalian intestine is a self-renewing organ that processes ingested food, absorbing nutrients from a sea of biochemical, mechanical, and pathogenic insults. To understand the developmental specification of diverse cell types in the small intestine, we performed time-resolved single-cell transcriptomics across five stages intestinal development: embryonic days (E) 12.5, 14.5, 16.5, and 18.5, and postnatal day (P) 1.5. By dissociating cells from whole organ preparations, we captured the full range of cellular diversity across all intestinal cell lineages. This allowed us to reconstruct the mesenchymal differentiation trajectory and identify the specific genetic programs that give rise to the diverse musculature of the gut wall and villus stroma. Time-resolved single-cell transcriptomics across five stages during intestinal development: embryonic days (E) 12.5, 14.5, 16.5, and 18.5, and postnatal day (P) 1.5.哺乳动物肠道是一类具有自我更新能力的器官，负责消化摄入的食物，并在充满生化、机械及病原性刺激的复杂环境中吸收营养物质。为阐明小肠内多种细胞类型的发育特化机制，我们针对肠道发育的五个阶段开展了时间分辨率单细胞转录组分析：胚胎日（E）12.5、14.5、16.5、18.5天，以及出生后日（P）1.5天。本研究通过解离完整器官制备物中的细胞，捕获了覆盖所有肠道细胞谱系的全部细胞多样性，借此得以重构间充质细胞的分化轨迹，并鉴定出驱动肠壁肌肉组织与绒毛基质多样化形成的特异性遗传程序。针对肠道发育五个阶段的时间分辨率单细胞转录组分析覆盖以下时期：胚胎日（E）12.5、14.5、16.5、18.5天，以及出生后日（P）1.5天。