Determination of affinity of TSHR for TSH in presence of TSHR MAbs.
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The membrane preparation (20 µg/ml) obtained from HEK293-hTSHR cells were incubated with either 50 µg/ml of NMIgG or TSHR MAbs. 125I-hTSH (2×105 cpm) was added to TSHR MAb –TSH complex in presence of increasing concentration of hTSH and Kd and Bmax value obtained by fitting the data as mentioned in the legends of Figure 3.apbp
创建时间：
2015-12-02
