Additional file 1 of RGG1, Involved in the Cytokinin Regulatory Pathway, Controls Grain Size in Rice

Additional file 1 : Fig. S1. Sequence alignment of the Gγ proteins in rice, Arabidopsis and maize. The red line indicates the predicted nuclear localization signal sequence. The red box indicates the CaaX isoprenylation motif at the C-terminal end. The green line indicates the GGL domain. Fig. S2. Subcellular localization of RGG1ΔNLS-GFP. Scale bar, 20 μm. Fig. S3. Internode lengths of NIP and the RGG1 OE lines. Scale bar, 5 cm. Fig. S4. Comparison of plant and grain phenotypes between WYJ30 and the OE lines. Fig. S5. Targeted mutagenesis of RGG1 in the WYJ30 background and protein sequence alignment of WYJ30 and the mutants. Fig. S6. Gene Ontology (GO) DEG enrichment of the biological process, molecular function and cellular component categories. Fig. S7. Relative expression levels of genes concerning cytokinin biosynthesis. Fig. S8. NaCl treatment of NIP and the transgenic lines. Table S1. Comparison of major agronomic traits between NIP and the transgenic lines. Table S2. Comparison of major agronomic traits between WYJ30 and the transgenic lines. Table S3. Primers used in this study.