Neural Lineage Progression Controlled by a Temporal Proliferation Program along the Apical-basial Axis

This project is focused on understanding how the early driver factors act along the A-P axis. We have recently found that neural stem cells in the brain divide for longer, and bud off daughters that divide more, hence generating larger lineages that those found in the nerve cord. Intriguingly, the early factors are expressed at a higher level in the brain, and her functional studies indicate that this provides a driving input that contributes to the generation of the large brain lineages. We generated quadruple UASs combination: ase, Kr;SoxN, wor (denoted UAS-Quad), and and sextuple UASs combination: hb, ase, Kr ; SoxN, nub, wor (denoted UAS-Hexa). To get the transcriptomic view of the effects of the Quad and Hexa misexpressions, we have run RNAseq analysis for to two separate set of experiments: 1 (control VS UAS-Quad) and 2(Control VS UAS-Hexa). UAS-Quad and UAS-Hexa misexpressions were driven from Insc-Gal4, and for the control, we have used the heterozygous Insc-Gal4 over plus. Within each experiment, we have three biological replicates per genotype (control and misexpressions). In total, we have had 9 samples (3 per each experiment).