Tumor vascular heterogeneity and the impact of sub-tumoral nanoemulsion biodistribution - Supplementary dataset

Aim: Investigate the heterogeneous tumor tissue organization and examine how this condition can
interfere with the passive delivery of a lipid nanoemulsion in two breast cancer preclinical models
(4T1 and Ehrlich). Materials & methods: The authors used in vivo image techniques to follow the
nanoemulsion biodistribution and microtomography, as well as traditional histopathology and electron
microscopy to evaluate the tumor structural characteristics. Results & conclusion: Lipid nanoemulsion
was delivered to the tumor, vascular organization depends upon the subtumoral localization and this
heterogeneous organization promotes a nanoemulsion biodistribution to the highly vascular peripherical
region. Also, the results are presented with a comprehensive mathematical model, describing the
differential biodistribution in two different breast cancer models, the 4T1 and Ehrlich models.