Modified protocols used for the extraction of RNA from archived bone marrow aspirate slides.

The modified extraction protocols used in this study for the extraction of total RNA and small RNA from unstained archived bone marrow aspirate slides. Two main methods were used, the Roche High Pure miRNA Isolation Kit and TRIzol. We followed two provided extraction protocols for the kit; ‘miRNA isolation from tissue’, and ‘miRNA isolation from FFPE tissue’. The methods provided by the manufacturers were modified to include a slide scraping step, and a digestion/homogenization step to break down the slide material. The kit allows for the extraction of total RNA as well as small RNA only; both these methods were trialled here; samples listed in Table S3.