Chemistry data for the exposure waters and tissue samples collected during the Madison County crayfish laboratory studies.

Historical lead mining in Madison County, Missouri, USA has left a legacy of metals contamination. Laboratory studies were conducted to examine the effect of water hardness and age of crayfish on the toxicity of environment-based metals mixture. Additionally, the effects of chronic exposure to a metals mixture to egg development in adult crayfish were assessed. These data are comprised of measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead), major anions (e.g., chloride, sulfate), dissolved organic carbon, and major cations (e.g, sodium, calcium, magnesium) in water samples collected during laboratory-based diluter exposure studies. Measurements of elements (i.e., cobalt, nickel, copper, zinc, cadmium, lead) in tissue samples (crayfish whole bodies, ovaries, leaves, feed) collected during laboratory-based diluter exposure studies are also provided.