Glutamate sensor and calcium signals in dopamine neurons and dopamine release

We recorded glutamate input to dopamine neurons in the ventral tegmental area (VTA) with iGluSnFR(A184S), a glutamate sensor, somatodendritic dopamine neuron activity in the VTA with jGCaMP7f, a calcium indicator, and dopamine release at the ventral striatum (VS) with GrabDA2m, a dopamine sensor, using fiber fluorometry. Recordings were performed in two types of reward association tasks (classical conditioning and sequential conditioning), with presentation of aversive airpuff to the eye with or without buprenorphine treatment, and with optogenetic activation of glutamate input axons at the VTA., Behavior
After 1 week of recovery from surgery, mice were water-restricted in their cages. All conditioning tasks were controlled by a NIDAQ board and LabVIEW. Mice were handled for 2 days, acclimated to the experimental setup for 1-2 days including consumption of water from the tube, and head-fixed with random interval water for 1-3 days until mice showed reliable water consumption. For odor-based classical conditioning, all mice were head-fixed, and the volume of water reward was constant for all reward trials (predicted or unpredicted) in all conditions (6 ml). Some sessions included mild air puff trials, directed at one of the eyes and the intensity of air puff was constant for all air puff trials (predicted or unpredicted; 2.5 psi). In classical conditioning, each association trial began with an odor cue (lasting 1 s) followed by a 2 s delay, and then an outcome (either water, nothing, or air puff) was delivered. In sequential conditioning, each association trial began with a dista..., , # Glutamate sensor and calcium signals in dopamine neurons and dopamine release

The recording data for glutamate input to dopamine, somatodendritic dopamine activity, and dopamine release at the ventral striatum. The datasets are divided into 5 different task conditions: ‘Classical_conditioning’, ‘Sequential conditioning’, ‘Airpuff_response’, ‘Airpuff_with_Buprenorphine’, and ‘Optogenetic_stimulation’.

## Description of the data and file structure

**File names**

Data of each recording session is stored in each “.mat” file (**_********.mat).

Task type is indicated at the beginning of the file name (‘task type’******.mat).

The mouse ID number is indicated in the middle of the file name (**_***’mouse ID’***.mat).

The session number is indicated at the end of the file name (**_**_**_session’session number’.mat). Task structures were exactly the same in each session, and each session was performed on different days.

The block name is indicated at the end of the file name after the se...