Catherine I. Lacayo, Zachary Pincus, Martijn M. VanDuijn, Cyrus A. Wilson, Daniel A. Fletcher, Frank B. Gertler, Alex Mogilner, Julie Theriot (2011) CIL:12087, Hypsophrys nicaraguensis, keratocyte. CIL. Dataset

Nascent Lamellipodia Emerge with Rough Edges and Rapidly Acquire a Smooth Morphology. A glass micropipette, acting as a barrier, was lowered into the path of movement of a migrating, coherent keratocyte overexpressing EGFP-VASP (an indicator of polymerizing actin). When the keratocyte reaches the barrier, the lamellipodium temporarily stops protruding forward, while EGFP-VASP becomes displaced from the edge of the region in contact with the barrier. When the micropipette barrier is removed (between 0:57 and 1:00), the leading edge of the lamellipodium immediately resumes protrusion and appears rough with several protruding microregions enriched in EGFP-VASP. Only 18 s after removal of the barrier, the keratocyte’s original shape and uniform EGFP-VASP localization at the leading edge are restored. Time ¼ min:s. Scale bar 25 micron. Corresponds to video S2 in PLos Biology 5:2035-2052, 2007.

初生伪足边缘粗糙，迅速获得光滑形态。通过将玻璃微吸管作为障碍物，降低至过表达EGFP-VASP（聚合肌动蛋白的指示剂）的迁移性、连续的角质细胞的运动路径中。当角质细胞触及障碍物时，伪足的前端暂时停止突出，同时EGFP-VASP从接触障碍物的区域边缘移位。当微吸管障碍物被移除（介于0:57至1:00之间）时，伪足的前端立即恢复突出，并呈现出粗糙的外观，伴随着富含EGFP-VASP的几个突出微区。仅在移除障碍物后的18秒内，角质细胞的原始形状及其在伪足前端的均匀EGFP-VASP定位得以恢复。时间标记为每分钟的四分之一：秒。比例尺为25微米。对应于PLos Biology 2007年第5卷第2035-2052页中的视频S2。