Transcriptomics E-Cigarette Exposure

To gain insights into repair processes and potential long-term consequences of acute EV exposure on lung health, hamsters were exposed to EV aerosols at concentrations shown to simulate human exposure via daily nasal nebulization for 5 consecutive days. To evaluate persistent pulmonary effects of a relatively brief EV aerosol exposure, animals were allowed a 10-day recovery period, with lung tissue harvested on day 15. In a separate experiment, we exposed hamsters to daily EV for 5 days, followed by SARS-COV-2 (3 days later); or, sequentially, to both IAV (the following day) and SARS-COV-2 (3 days later). Lungs were harvested on day 7 after SARS-CoV-2 infection (during the injury phase). This experimental design also enabled us to test the effect of IAV pre-infection on host responses to SARS-CoV-2, with or without EV exposure. Data uploaded are gene counts from bulk RNA sequencing performed by Novogene.