Changes in mRNA nucleocytoplasmic distribution in response to nitrate in Arabidopsis thaliana roots

Nitrate, the primary source of nitrogen in agricultural soils, is an important signaling molecule that regulates global gene expression in plants. The nitrate response has been extensively characterized at the transcriptome level using RNA from complete organisms, organs or cells. However, we know very little about the subcellular localization of mRNA or its impact on gene expression. To understand the nucleocytoplasmic dynamics of transcripts during the nitrate response, we isolated mRNA from the nucleus, cytoplasm, and whole-cells from nitrate-treated Arabidopsis thaliana roots and performed RNA-seq analysis. These data show a dynamic mRNA nucleocytoplasmic distribution in response to nitrate and allows to understand the role for RNA localization in the plant's adaptive response to nitrogen nutrient signals.