Effects of TRIM33 knockdown on ER cistrome and transcriptome in breast cancer cells

Estrogen receptor alpha (ER) is a nuclear hormone receptor responsible for estrogen-driven breast tumor growth. ER transcriptional activity is modulated by interactions with coregulators. Dysregulation of the levels of these coregulators is involved in the development of endocrine resistance. Mass spectrometry analysis revealed tripartite motif containing 33 (TRIM33) as an estrogen-dependent interactor of ER. shRNA knockdown showed that TRIM33 promoted ER transcriptional activity.