Gene expression profiling of the mandible in mice with a conditional inactivation of Erk2 at embryonic day E12.5

We performed global gene expression analyses of the mandible in Wnt1-Cre;Erk2^fl/fl and Erk2^fl/fl control mice at embryonic day E12.5. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the Enzo BioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip® Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39000 transcripts. Microarrays were hybridized, processed and scanned as previously described using manufacturers recommended conditions.