Table 1 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes

Table 1 Summary of different responses of zygotic immature embryos to different basal medium (BM1 and BM2), combination and concentration of plant growth regulators (PGRs), and light and dark conditions.The response of explants was in the form of callus (C), somatic embryogenesis (SE), and adventitious shoot (AS).The Basal media were BM1: MS mineral salts,B5 vitamins, 30 g L − 1 sucrose, BM2: half strength MS macro salts, B5 vitamins, 100 mg L − 1 L-glutamine, 500 mg L − 1 casein hydrolysate, and 30 g L − 1 sucrose.
CodingPGRs combinations (mg L − 1)LightDarkBM1BM2BM1BM2BM1BM2BM1BM2BM1BM2BM1BM2CSEASCSEASE10–––+––––––––E20.01 IBA–––+–––+––––E30.01 IBA + 1 BA–––––+–+––––E40.01 IBA + 2 BA–––––+–+–+––E50.01 IBA + 3 BA–––––+–+––––E60.01 2,4-D–––+–––+––––E70.01 2,4-D + 0.1 TDZ–––––––+––––E80.01 2,4-D + 0.25 TDZ–––––––+––––E90.01 2,4-D + 0.5 TDZ–––––––+––––E100.01 2,4-D + 1 TDZ–––––––+––––E110.01 NAA–––+–––+––––E120.01 NAA + 1 BA–––––+–+–––+E130.01 NAA + 2 BA–––––+–+–––+E140.01 IBA + 0.1 TDZ–––––+–+––––E150.01 IBA + 0.25 TDZ–––––+–+––––E160.01 IBA + 0.5 TDZ–––––+–+––––E170.01 IBA + 1 TDZ–––––+–+–+––
«–» and « + » represent nonexistence and existence the response on explants that cultured in intended media, respectively.