File S1 - Characterization of a Self-sufficient Trans-Anethole Oxygenase from Pseudomonas putida JYR-1

Contans: Figure S1. Schematic diagram of partially deleted GST-TAO. Figure S2. Optimal reaction temperature (A) and pH (B) conditions for GST-TAO activity in the presence of NADH and FAD. For the optimal pH conditions, purified GST-TAO was incubated with different buffers, 100 mM of sodium acetate buffer (pH 4.0–5.8) (▪), potassium phosphate (pH 6.2–8.0) (•), Tris-Cl buffer (pH 8.0–9.0) (▴), and glycine-NaOH buffer (pH 9.0–10.6) (▾) at 30°C for 60 min incubation time. Figure S3. Stability of GST-TAO after prolonged incubation at 25°C. (DOC)