The structure and allelic diversity of the self-incompatibility locus (S-locus) in diploid potatoes inferred from genome sequences and transcriptome data from styles and pollen

Gametophytic Self-Incompatibility is a reproductive strategy to prevent inbreeding and promote outcrossing, and it is controlled by the self-incompatibility locus (S-locus). Studies to understand molecular and evolutionary aspects of the SI system in the Solanaceae have been conducted using several genera, including Petunia, Nicotiana, and Solanum. S-RNases are pistil determinants of gametophytic SI, and multiple S-RNase alleles have been identified in a few potato species. SLFs, the pollen determinants of SI, are linked to S-RNases on chromosome 1. The S-RNase and SLFs present on each chromatid determine an individual’s self-compatible (SC) or self-incompatible (SI) haplotypes. We used long-read genome sequencing and RNA sequencing from styles and pollen to assess the diversity and composition of elements within the S-locus. RNA for RNA-seq was collected from stylar tissue and germinated pollen. For three genotypes, PacBio Iso-Sequencing was used to confirm the presence of alternative ..., , # Data from: The structure and allelic diversity of the self-incompatibility locus (S-locus) in diploid potatoes inferred from genome sequences and transcriptome data from styles and pollen

Dataset DOI: [10.5061/dryad.qz612jmv1](https://doi.org/10.5061/dryad.qz612jmv1)

## Description of the data and file structure

#### Files and variables

**File: Genomes.zip**

**Description:** contains assembled phased genomes in fasta format and annotation files in gff format. Fasta files were generated from PacBio HiFi data and assembled using Hifiasm v. 0.19.9. Gene prediction was done using Augustus v.3.5.0. Each sub-folder inside the Genomes folder is dedicated to a specific genotype. The identity of each WiDiPo genotype is listed in the genotypes_list.txt file.

**File: Style_RNAseq.zip**

**Description:** contains assembled style transcriptomes in fasta format and classifications of transcripts based on functional annotations of T2-Ribonucleases (GO0033887) as DNA and protein sequences. Also...,