Single-cell analyses offer insights into the different remodeling programs of arteries and veins (scRNA-Seq)

We compared 3 non-diseased human arteries (brachial) and 3 veins (2 basilic, 1 cephalic) by single cell RNA sequencing and histology. Vessels were collected in cold cell culture media and enzymatically digested to generate single-cell suspensions. Samples were run using the Chromium Single Cell 3′ Library & Gel Bead Kit v3 (10X Genomics) with a target capture of 10,000 cells or as indicated otherwise. Libraries were sequenced on the Illumina NovaSeq 6000. FASTQ files were generated with Cell Ranger's mkfastq pipeline (version 6.0.2). The Cell Ranger's count pipeline (version 6.0.2) was used to generate raw gene-barcode matrices from alignment to the 10X Genomics pre-built Cell Ranger human reference package (version 2020-A), from GRCh38 Ensembl build 98/GENCODE v32 gene annotations.