Fun30-Myc ChIP-seq in Spo11 wild type and spo11-yf mutants. Fun30-Myc ChIP-seq in Spo11 wild type and spo11-yf mutants

Resection, nucleolytic processing of DSB ends is necessary to generate 3' single-stranded DNA tails (ssDNA) for homologous recombination (HR). Meiotic recombination initiated by Spo11 induced double-strand breaks (DSBs) is essential for the accurate segregation of homologous chromosomes. After cleavage, Spo11 stays covalently linked to the DSB ends, which requires MRX/Sae2 incision on broken molecules to allow following Exo1-mediated resection. Exonuclease I activity is inhibited by nucleosome bound DNA in vitro. To ensure resection proceeds, resection machineries must overcome chromatin barrier. Here we show that DSB dependent enrichment of Fun30 proteins at hotspots and meiotic axes. Overall design: 16 samples total: two biological replicate ChIP-seq of S.cerevisiae for each genetic background and at 4h after meiosis induction