Shotgun lipidomics of mouse liver with ASO mediated PNPLA3 knockdown in WT mice stimulated with LXR agonist 'T0901317'.

10-week-old female wild-type C57BL/6J mice were ad-libitum fed a corn-oil enriched Western Diet, a modified Western diet with corn oil substituted for butter (D21050712i) matched to D12079B (Research Diets, New Brunswick, NJ). During 3 week feeding time course, mice were administered 10 mg/kg ASO in PBS twice a week via IP injection. For LXR agonist administration, both groups of mice were injected 10 mg/kg T0901317 in 1:1 m-pyrrole: ethanol (5 mg/ml) on day 19 and day 20. Following metabolic synchronization, mice were sacrificed after 6 h of refeeding on day 21.Total lipids were extracted from mouse liver tissues and analyzed using multidimensional mass spectrometry (MDMS)-based shotgun lipidomics (MDMS-SL) at the University of Texas Health Science Center at San Antonio, as previously detailed (Yang et al., Anal.Chem., 2009)Control ASO group: 05, 06, 07PNPLA3 ASO group: 08, 09, 10