Analysis of differential gene expression in the choroid plexus (ChP) of P60 mice at 9am and 9pm

3 mice per timepoint (9am and 9pm) Cerebrospinal fluid (CSF) provides vital support for the central nervous system (CNS). Abnormal CSF components have been associated with a number of disease states including conditions with disrupted circadian rhythmicity like Alzheimer's disease and bipolar disorder. The choroid plexus (ChP) is specialized to secrete CSF and displays circadian rhythmicity, but how the output of this tissue changes thorough the day to alter the CNS environment is unknown. In other tissues, protein synthesis rates often vary across time of day. Using ribosomal profiling, we identified translation and metabolism as downstream of the circadian clock in the ChP. Further, we identify ChP secreted factors that are differentially regulated by translation and dependent on core circadian machinery and feeding for this differential expression. These results provide insight into changing ChP circadian output that could dramatically change the CNS environment throughout the day. TRAP mRNAs were isolated and sequenced from the choroid plexus (ChP) of P60 mice每个时间点（上午9时与晚间9时）使用3只小鼠。脑脊液（Cerebrobrospinal fluid, CSF）为中枢神经系统（Central Nervous System, CNS）提供关键支持。脑脊液成分异常与多种疾病状态相关，涵盖昼夜节律紊乱相关病症，例如阿尔茨海默病与双相情感障碍。脉络丛（Choroid plexus, ChP）具备分泌脑脊液的特化功能，并呈现昼夜节律性，但目前尚不明确该组织的分泌输出如何随昼夜更替改变中枢神经系统微环境。在其他组织中，蛋白质合成速率通常随一日内的不同时段发生波动。本研究通过核糖体谱分析（ribosomal profiling），鉴定出脉络丛中受昼夜节律时钟下游调控的翻译与代谢过程。此外，本研究还鉴定出受翻译调控的脉络丛分泌因子，且这些因子的差异表达依赖核心昼夜节律机制与进食行为。上述研究结果为揭示脉络丛昼夜分泌输出的动态变化提供了关键见解，而该变化可在全天内显著改变中枢神经系统微环境。本研究从出生后60日龄（P60）小鼠的脉络丛中分离并测序了TRAP mRNA。