High-throughput explorations of Drosophila models of AKT1 variants associated with juvenile ovarian granula cell tumors provide insights into their pathogenesis mechanisms.

To investigate the effect of AKT1 mutants discovered in juvenile granulosa-cell tumors, we generated UAS-AKT1 transgenic flies (Drosophila melanogaster), expressing either the human wild type or a mutant form of AKT1. Next, we performed high-throughput analyses of the transcripts deregulated after mild over-expression of human WT and mutated AKT1 variants in Drosophila follicular cells. Site-specific transformation of pUASTattB-AKT1-3xFLAG constructs into the 51C fly line (y1 M{vas-int.Dm}ZH-2A w*; M{3xP3-RFP.attP'}ZH-51C)(Reference BDSC Stock # 24482) was performed by Bestgene, Inc. (CA, U.S.A.). Comparative of genes dysregulated in follicular cells expressing mutant form of AKT1 compared to WT AKT1 or empty Landing pad (#24482 fly stock) after RNAseq experiment