Induction of neutralizing antibodies against EV71 E59 and 5746 strains and CVA16 by Ad-EVVLP or FI-EV71 vaccine.

Seven-week-old BALB/c mice were individually primed and boosted at 14-day intervals i.p., s.c., or orally with 108 pfu Ad-EVVLP or Ad-LacZ. Sera collected on Day 21 were analyzed for neutralizing activity by incubating 102 pfu 5746, E59 or CVA16 with varying dilutions of individual immune sera before being added to RD cells. CPE was observed after 5 days of culture. The results were expressed as neutralizing titers that correspond to the dilution of immune sera, giving TCID50 value of 50% reduction of cytopathic effect.Induction of neutralizing antibodies against EV71 E59 and 5746 strains and CVA16 by Ad-EVVLP or FI-EV71 vaccine.