Genome-wide identification of transcriptional targets for H3K23ac, H3K27ac, and H4K5ac in vascular smooth muscle cells of aneurysmal aortas [CUT&Tag]

In this study, we found that H3K23ac, H3K27ac, and H4K5ac level are elevated in VSMCs of aneurysmal aortas. To further explore the potential functional significance of H3K23ac, H3K27ac, and H4K5ac in aortic aneurysm, we performed genome-wide cleavage under targets and tagmentation (CUT&Tag) analysis to identify candidate genes regulated by H3K23ac, H3K27ac, or H4K5ac in rat VSMC. Following CUT&Tag, H3K23ac, H3K27ac, or H4K5ac-associated DNAs were amplified using non-biased conditions, labeled, and sequenced with Illumina NovaSeq 150PE.本研究发现，动脉瘤主动脉的血管平滑肌细胞（vascular smooth muscle cells, VSMCs）中H3K23ac、H3K27ac及H4K5ac的修饰水平均升高。为进一步探究H3K23ac、H3K27ac及H4K5ac在主动脉瘤中的潜在功能意义，本研究开展了全基因组靶向切割与标签化（cleavage under targets and tagmentation, CUT&Tag）分析，以鉴定大鼠VSMCs中受H3K23ac、H3K27ac或H4K5ac调控的候选基因。完成CUT&Tag实验后，研究人员采用无偏倚扩增条件对与H3K23ac、H3K27ac或H4K5ac结合的DNA进行扩增、标记，并使用Illumina NovaSeq 150PE平台完成测序。