A Wnt-Bmp feedback circuit controls inter-tissue signaling dynamics in tooth organogenesis

Gene expression profiling of the epithelial and mesenchymal compartments of the developing mouse tooth using Laser Capture Microdissected (LCM) or enzymatic treatment with Dispase to isolate the dental epithelial and mesenchymal tissue, over a developmental period from E10.0-E14.5. The data is also accessible from http://compbio.med.harvard.edu/ToothCODE/ The aim of the study is to investigate the the mode of epithelial-mesenchymal (E-M) interaction through analyzing global gene expression patterns. We collected wildtype spatiotemporal gene expression data and perturbation data from transcription factor knockouts and signaling molecule treatments to identify differentially regulated genes and reconstruct a gene regulatory network controlling E-M interaction in early tooth development.本数据集针对胚胎发育阶段为E10.0至E14.5的小鼠牙齿，采用激光捕获显微切割（Laser Capture Microdissection, LCM）或分散酶（Dispase）酶解处理的方法分离牙上皮与间充质组织，并对其上皮和间充质区域开展基因表达谱分析。该数据集可通过http://compbio.med.harvard.edu/ToothCODE/获取。本研究旨在通过分析全局基因表达谱，探究上皮-间充质（E-M）互作模式；我们收集了野生型时空基因表达数据，以及转录因子敲除与信号分子处理所得的扰动数据，以鉴定差异调控基因，并重构调控早期牙齿发育中E-M互作的基因调控网络。