Genome wide analysis of SAHA (vorinostat) treatment in primary mouse cortical neurons upon miR-203 overexpression

Purpose: The goal of this study was to assess gene expression changes upon SAHA treatment in cells overexpressing miR-203. Primary cortical cultures were established using E15 cortical cultures from C57BL/6J mice. At DIV0, cells were infectd with either miR-203 (high titre - 1MOI) or intermediate titre (0.5MOI) or control (high 1MOI) lentivirus andalso treated with different dose of SAHA. At DIV8, total RNA was isolated using NucleoSpin RNA XS kit (Takara). Libraries were prepared using Standard illumina stranded mRNA-seq protocol.