Mean and SEM values of cell membrane FRET efficiency and fraction of receptor components involved in FRET between the CaSR and RAMPs.

Significant difference in the NFRET value was observed between CaSR+RAMP1 and 3 vs. CaSR+RAMP2, and between CaSR+RAMP1 vs. CaSR +RAMP3 (***p<0.0001, Kruskal-Wallis test, Dunn's multiple comparison test). Significant differences were also observed in the fraction of RAMP3 and RAMP1 donor (Fd) in cell-surface FRET complex (*p<0.05, Mann Whitney test). There was no significant difference between fraction of CaSR acceptor in the FRET complex between RAMPs 1 and 3 (Fa). The table displays combined data of three independent experiments.