Fluorescent Protein Excitation and Emission.

Senor nomenclature is as follows: Zap refers to the 1st two zinc fingers of the Saccaromyces cerevisiae Zap1 transcription factor that serves as the zinc binding domain, the next two letters refer to the donor and acceptor FPs, finally the “1” at the end of a sensor name indicates the wild type Zap1 domain was used, the “2” indicates that two mutations (Cys to His) were incorporated to lower the zinc affinity, as outlined in [15]. “1.1” indicates one mutation (Cys to His) was incorporated.