Downregulation of MED12 via siRNA in 22Rv1 prostate cancer cells (full transcriptome)

The Mediator complex is a multi-subunit protein that modulates gene expression on a genome-wide scale. MED12 and cyclin-dependent kinase 8 (CDK8) or its paralog CDK19 are components of its kinase module that regulate the proliferation of prostate cancer cells. In this study, we investigated how MED12 and CDK8/19 influence cancer-driven processes in prostate cancer cell lines, focusing on AR activity and enzalutamide resistance. Overall design: To investigate the relevance of MED12 in de novo enzalutamide resistant 22Rv1 cells, we performed siRNA mediated knockdown and analyzed the response using differential gene expression analysis.