Functional Recovery from Human Pluripotent Stem-Derived Dopamine Neuron Grafts is Dependent on Neurite Outgrowth

We conducted bulk RNAseq to understand changes in the transcriptome of in vitro dopaminergic progenitor cells as their duration of differentiation changes. We obtained gene expression estimates from Day 13, (n=2), Day 18 (n=2), and Day 25 (n=2) cell cultures. Two Parkinson's Disease patients provided skin cells that were reprogrammed -> fibroblasts -> iPSC -> Dopaminergic neurons. At Days 13, 18 and 25 of dopeminergic neuron differentiation, cells were isolated for bulk RNA-seq. Paired-end RNA sequencing was performed on the Illumina HiSeq 2000 platform, with TruSeq_v2 library preparation chemistry.