Gene expression of yeast fermentor culture after circadian temperature entrainment

This gene expression experiment aimed to discover circiadian clock genes in yeast cells grown in a 1L fermentor culture Yeast were entrained for a period of time after the culture was released to a freerun (see growth protocol below). Samples were taken from fermenter cultures (1 × 109 yeast cells) every 3 hours over the first day of constant conditions (free-run), starting 1h before the temperature transition from 21°C to 28°C 9 Samples were taken from a fermenter culture every 3 hours over the first day of constant conditions (free-run), starting 1h before the temperature transition from 21°C to 28°C