Experiment on the cultivation of diatom-associated bacteria with the addition of 13C/12C-SQ.

Using 13C/12C-SQ as the substrate, after adding cultured diatom-associated bacteria, bacteria were collected and DNA was extracted. The extracted DNA underwent ultracentrifugation and was divided into 15 fractions based on density, with appropriate fractions selected for next-generation sequencing of 16S rRNA gene sequencing. For the total DNA of the bacteria collected before and after cultivation, third-generation 16S rRNA gene sequencing was performed. The cultivation samples included three biological replicates. The data labeled as A/B/C represent the three biological replicates of the 13C-SQ addition culture experimental group, while the data labeled as D/E/F represent the three biological replicates of the 12C-SQ addition control group. The number following the letter indicates which fraction of the sample was obtained after ultracentrifugation, for example, A1 represents the first fraction of sample A. Y represents the third-generation sequencing of the 16S rRNA gene from the total bacterial DNA before cultivation. Identifying marine bacteria capable of metabolizing SQ. The purpose of this study is to identify marine bacterium capable of metabolizing SQ